CEA Antibody / CEACAM5

CAT: 0013-GTR18572319Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18572319Size:100 µg

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Description

This antibody recognizes proteins of 80-200kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+) . CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

UniProt

P06731

Reactivity

Human

Immunogen

Human full-length recombinant CEA protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

C66/1030

Conjugation

Unconjugated

Field of Research

Cancer Biology; Cell Biology

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT (1) (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Clone C66/1030 (recommended capture) will pair with clone C66/1009 (recommended detect) by ELISA. It will NOT pair with clone C66/261.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

UniProtKB · P06731

Cell adhesion molecule CEACAM5

CEAM5_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P06731
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CEACAM5
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: H9KVA7

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCell adhesion

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Domain

Immunoglobulin domainRepeatSignal

Disease

Oncogene

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