SSPE Buffer 20X (Biotechnology Grade)

• Description:

  SSPE Buffer 20X (Biotechnology Grade)_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_

  Catalog number:	B2010087
  Lot number:	Batch Dependent
  Expiration date:	Batch Dependent
  Volume/Weight	50 mL
  pH	7.4
  Supplied as	20X concentrate (working concentration ranges between 0.1X and 20X depending on application)
  Appearance	Clear solution
  Applications	SSPE Buffer (20X) is a highly pure, filter-sterilized, reproducible buffer for useful for bacterial screening, hybridization procedures for nucleic acid and blot transfer applications. Working concentrations can be prepared by diluting the provided concentrate with di-water to a final concentration of 0.1X to 20X (depending on the application).
  Shelf-Life	1 year from date of manufacture
  Storage:	Keep at 2-8°C
  Keywords:	Sodium Chloride-Sodium Phosphate-EDTA Buffer, SSPE 20X, SSPE concentrate
  Grade	Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
  References	1: Goldau T, Murayama K, Brieke C, Asanuma H, Heckel A. Azobenzene C-Nucleosides_x000D_ for Photocontrolled Hybridization of DNA at Room Temperature. Chemistry. 2015_x000D_ Dec 1;21(49):17870-6.2: Markegard CB, Gallivan CP, Cheng DD, Nguyen HD. Effects of Concentration and_x000D_ Temperature on DNA Hybridization by Two Closely Related Sequences via Large-_x000D_ Scale Coarse-Grained Simulations. J Phys Chem B. 2016 Aug 18;120(32):7795-806.3: do Nascimento C, Muller K, Sato S, Albuquerque Junior RF. Effect of sample_x000D_ storage time on detection of hybridization signals in Checkerboard DNA-DNA_x000D_ hybridization. Can J Microbiol. 2012 Apr;58(4):502-6._x000D_ 4: Avarre JC, de Lajudie P, Béna G. Hybridization of genomic DNA to microarrays:_x000D_ a challenge for the analysis of environmental samples. J Microbiol Methods. 2007_x000D_ May;69(2):242-8._x000D_ 5: Cho JC, Tiedje JM. Bacterial species determination from DNA-DNA hybridization_x000D_ by using genome fragments and DNA microarrays. Appl Environ Microbiol. 2001_x000D_ Aug;67(8):3677-82._x000D_ 6: Su X, Wu YJ, Knoll W. Comparison of surface plasmon resonance spectroscopy_x000D_ and quartz crystal microbalance techniques for studying DNA assembly and_x000D_ hybridization. Biosens Bioelectron. 2005 Nov 15;21(5):719-26._x000D_ _x000D_ 7: Hu Y, Yang T, Li Q, Guan Q, Jiao K. Conjugated self-doped polyaniline-DNA_x000D_ hybrid as trigger for highly sensitive reagentless and electrochemical self-_x000D_ signal amplifying DNA hybridization sensing. Analyst. 2013 Feb_x000D_ 21;138(4):1067-74.
  Related Products	Biochemical Buffers and Solutions

• Short Description:

  Catalog Number: B2010087 (50 mL)

• Weight:

  2.6

• Length:

  3.3

• Width:

  1.2

• Height:

  1.2

• Height :

  1.2