Recombinant Meleagris gallopavo Acrosin (ACR)

• Product Name Alternative:

  Proacrosin

• Abbreviation:

  Recombinant Meleagris gallopavo ACR protein

• Gene Name:

  ACR

• UniProt:

  Q2UVH8

• Expression Region:

  20-346aa

• Organism:

  Meleagris gallopavo (Common turkey)

• Target Sequence:

  FSGGCDTCGLRPVAYHYGGMRVVGGTEALHGSWPWIVSIQNPRFAGTGHMCGGSLITPQWVLSAAHCFGRPNYILQSRVVIGANDLTQLGQEVEVRSIRRAILHEYFNNKTMINDIALLELDRPVHCSYYIQLACVPDPSLRVSELTDCYVSGWGHMGMRSAAPTQTAEVLQEAKVHLLDLNLCNSSHWYDGVLHSHNLCAGYPQGGIDTCQGDSGGPLMCRDSSADYFWLVGVTSWGRGCGRAFRPGIYTSTQHFYNWILLQVRAAAHPTSRTWSHYMSTSSYHHGPNAVPTQPSVSDSCPFPAQKLREFFTGVQNLLQSLWGSKA

• Tag:

  N-terminal MBP-tagged and C-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  Baculovirus

• Field of Research:

  Others

• Relevance:

  Serine protease of trypsin-like cleavage specificity. Synthesized in a zymogen form, proacrosin and stored in the acrosome

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Serine protease of trypsin-like cleavage specificity. Synthesized in a zymogen form, proacrosin and stored in the acrosome (By similarity) .

• Molecular Weight:

  80.1 kDa

• References & Citations:

  "Acrosin activity in turkey spermatozoa: assay by clinical method and effect of zinc and benzamidine on the activity." Glogowski J., Jankowski J., Faruga A., Ottobre J.S., Ciereszko A. Theriogenology 56:889-901 (2001)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein