Products for Research Use Only

Recombinant Shigella flexneri Glutaredoxin-4 (grxD)

CAT: 0399-CSB-EP364758SZB-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP364758SZB-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Monothiol glutaredoxin (ydhD) (Grx4)
Abbreviation
Recombinant Shigella flexneri grxD protein
Gene Name
GrxD
UniProt
P0AC72
Expression Region
1-115aa
Organism
Shigella flexneri
Target Sequence
MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACGERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKETAAKYKSEEPDAE
Tag
N-terminal 6xHis-SUMO-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Cell Biology
Relevance
Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters.
Molecular Weight
28.9 kDa
References & Citations
"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786 (2003)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length

UniProtKB · P0AC72

Glutaredoxin 4

GLRX4_SHIFL · Shigella flexneri

View on UniProt ↗
Primary accession
P0AC72
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
grxD
Protein existence
3: Inferred from homology
Organism
Shigella flexneri
Taxonomy ID
623
Alternative names
Grx4
EC number
—
Processing
—
Secondary accessions
P37010, P77424
Protein keywords

Ligand

2Fe-2SIronIron-sulfurMetal-binding

Cellular component

Cytoplasm

Domain

Redox-active center

Technical term

Reference proteome