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Recombinant Mouse Alpha-enolase (Eno1), partial

CAT: 0399-CSB-EP007670MOb0-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP007670MOb0-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
2-phospho-D-glycerate hydro-lyase Enolase 1 Non-neural enolase
Abbreviation
Recombinant Mouse Eno1 protein, partial
Gene Name
Eno1
UniProt
P17182
Expression Region
2-433aa
Organism
Mus musculus (Mouse)
Target Sequence
SILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLA
Tag
N-terminal 10xHis-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Signal Transduction
Relevance
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (By similarity) . May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production (By similarity) .
Molecular Weight
50.4 kDa
References & Citations
"Cholesteryl ester loading of mouse peritoneal macrophages is associated with changes in the expression or modification of specific cellular proteins, including increase in an alpha-enolase isoform." Bottalico L.A., Kendrick N.C., Keller A., Li Y., Tabas I. Arterioscler. Thromb. 13:264-275 (1993)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial

UniProtKB · P17182

Alpha-enolase

ENOA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P17182
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Eno1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
4.2.1.11
Processing
—
Secondary accessions
Q99KT7, Q9DCY7
Protein keywords

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Cellular component

Cell membraneCytoplasmMembrane

Technical term

Direct protein sequencingReference proteome

Biological process

Glycolysis

Molecular function

Lyase

Ligand

MagnesiumMetal-binding