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Recombinant Cynodon dactylon Profilin (PRO1)

CAT: 0399-CSB-EP517332EQB-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP517332EQB-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Pollen allergen Cyn d 12 Allergen: Cyn d 12
Abbreviation
Recombinant Cynodon dactylon PRO1 protein
Gene Name
PRO1
UniProt
O04725
Expression Region
2-131aa
Organism
Cynodon dactylon (Bermuda grass) (Panicum dactylon)
Target Sequence
SWQAYVDDHLMCEIEGHHLTSAAIIGHDGTVWAQSAAFPAFKPEEMANIMKDFDEPGFLAPTGLFLGPTKYMVIQGEPGAVIRGKKGSGGVTVKKTGQALVIGIYDEPMTPGQCNMVIEKLGDYLIEQGM
Tag
N-terminal 6xHis-SUMO-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Allergen
Relevance
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity) .
Molecular Weight
30 kDa
References & Citations
"Cloning and high level expression of Cynodon dactylon (Bermuda grass) pollen profilin (Cyn d 12) in Escherichia coli: purification and characterization of the allergen."Asturias J.A., Arilla M.C., Gomez-Bayon N., Martinez J., Martinez A., Palacios R.Clin. Exp. Allergy 27:1307-1313 (1997)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein

UniProtKB · O04725

Profilin

PROF_CYNDA · Cynodon dactylon

View on UniProt ↗
Primary accession
O04725
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRO1, PRO2
Protein existence
1: Evidence at protein level
Organism
Cynodon dactylon (Bermuda grass)
Taxonomy ID
28909
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Molecular function

Actin-binding

Disease

Allergen

Cellular component

CytoplasmCytoskeleton

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