Recombinant Xanthomonas campestris pv. campestris NAD-dependent protein deacetylase (cobB)

• Product Name Alternative:

  Regulatory protein SIR2 homolog

• Abbreviation:

  Recombinant Xanthomonas campestris pv. campestris cobB protein

• Gene Name:

  CobB

• UniProt:

  B0RM75

• Expression Region:

  1-293aa

• Organism:

  Xanthomonas campestris pv. campestris (strain B100)

• Target Sequence:

  MTVAITQTGPALQEFIKRHQRLFVLSGAGCSTDSGIPDYRDLHGGWKRPQPVTFQAFMGELSTRQRYWARSLVGWPRFGLARPNATHHALAALEARGQLELLLTQNVDRLHQAAGSQAVIDLHGRLDVVRCMGCEQRMPRTEFQLLLERDNPGWADLEAAQAPDGDADLDSVAFDNFVVPACPACGCVLKPDVVFFGENVPRERVERAFAHLQAADAVLVVGSSLMVYSGFRFVQAAARAGLPIAALNFGRTRADDLLSLKVEQSCAQALAFLQQPPDPLHTATARYHSARSA

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form.

• Molecular Weight:

  48.1 kDa

• References & Citations:

  "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.J. Biotechnol. 134:33-45 (2008)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length