Recombinant Lemna minor ATP synthase subunit beta, chloroplastic (atpB)

• Product Name Alternative:

  ATP synthase F1 sector subunit beta

• Abbreviation:

  Recombinant Lemna minor atpB protein

• Gene Name:

  AtpB

• UniProt:

  A9L9A3

• Expression Region:

  1-497aa

• Organism:

  Lemna minor (Common duckweed)

• Target Sequence:

  MQINPTTSGTAVSQLEEKNLGRVAQIIGPVLDVVFPPGKMPNIYNALVVKGQDADGQEIKVTCEVQQLLGNNRVRAVAMSATDGLTRGMDVIDTGAPLSVPVGGATLGRIFNVLGEPVDNLGPVDTRTTSPIHRSAPAFIQLDTKLAIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVINEKNITESKVALVYGQMNEPPGARMRVGLTALTMAEYFRDVNEQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLSTEMGSLQERITSTKEGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLAAKGIYPAVDPLDSTSTMLQPGIVGEDHYETAQRVKETLQRYKELQDIIAILGLDELSEEDRLTVARARKIERFLSQPFFVAEVFTGSPGKYVGLEETIRGFKLILSGELDSLPEQAFYLVGNIDEATAKAINLEVESKLK

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Produces ATP from ADP in the presence of a proton gradient across the mbrane. The catalytic sites are hosted primarily by the beta subunits.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.

• Molecular Weight:

  57.5 kDa

• References & Citations:

  Complete sequence of the Duckweed (Lemna minor) chloroplast genome structural organization and phylogenetic relationships to other angiosperms.Mardanov A.V., Ravin N.V., Kuznetsov B.B., Samigullin T.H., Antonov A.S., Kolganova T.V., Skyabin K.G.J. Mol. Evol. 66:555-564 (2008)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length