Recombinant Mouse C-C motif chemokine 12 (Ccl12)

• Product Name Alternative:

  MCP-1-related chemokine; Monocyte chemoattractant protein 5Monocyte chemotactic protein 5 ; MCP-5Small-inducible cytokine A12

• Abbreviation:

  Recombinant Mouse Ccl12 protein

• Gene Name:

  Ccl12

• UniProt:

  Q62401

• Expression Region:

  23-104aa

• Organism:

  Mus musculus (Mouse)

• Target Sequence:

  GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Chotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

• Molecular Weight:

  13.3 kDa

• References & Citations:

  Distinct expression and function of the novel mouse chemokine monocyte chemotactic protein-5 in lung allergic inflammation.Jia G.-Q., Gonzalo J.A., Lloyd C., Kremer L., Lu L., Martinez A.C., Wershil B.K., Gutierrez-Ramos J.-C.J. Exp. Med. 184:1939-1951 (1996)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein