Recombinant Bacteroides vulgatus Alpha-1,3-galactosidase B (glaB1)

• Product Name Alternative:

  Exo-alpha-galactosidase B1 (EC:3.2.1.22)

• Abbreviation:

  Recombinant Bacteroides vulgatus glaB1 protein

• Gene Name:

  GlaB1

• UniProt:

  A6KWM0

• Expression Region:

  15-592aa

• Organism:

  Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

• Target Sequence:

  CTTLSAQKVYEISAFGLKANSSKNASPVLQKALAKIKAEYKEGEKVILRFPEGRYEFHEKGAAVREYYISNHDQTNPKKVGIALEDMKNLTLDGQGSEFVFHGRMLPVSLLRSENCLLKNFSIDFENPHIAQVKIVENDPQDGIVFEPAPWVDYRIAKDSIFEAYGEGWTMRHSWGIAFDGDTKHLVYNTSDIGCPTKGASEVAPRRIHAPGWKDARLVPGTVVAMRGWGRPTPGIFLSHDVNTTIENVKVHYAEGMGLLAQLCENITLEKFGVCLKGDADPRYFTTQADATHFSGCKGKIVSCNGLYEGMMDDAINVHGTYLKVVKRVDDRTLVGRYMHGQSWGFEWGCPGDEVQFIRSNTMELVGKQNKIISIRPYDKEQTEGAREFLITFQEPVDQVINEQSGFGIENLTWTPEVLFSGNVIRNNRARGSLFSTPRKTIVENNLFDHTSGAAILLCGDCNGWFETGACRHVIIRKNRFVNALTNLFQFTNAVISIYPEIPDLKGQQQYFHGGPEGGIVIEDNEFETFDAPILYAKSVDGLVFRNNTIKLNTEYKPFHPNRNRFWLERVTNVTIAE

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Alpha-galactosidase. Roves both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures .

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures.

• Molecular Weight:

  69 kDa

• References & Citations:

  Evolution of symbiotic bacteria in the distal human intestine.Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I.PLoS Biol. 5:1574-1586 (2007)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein