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Rat SCF Protein

CAT: 0749-PRP1240-01Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1240-01Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Stem cell factor (SCF), as a key ligand of KIT, regulates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Binding to KIT activates signaling pathways involving phosphorylation events, key kinases (AKT1, RAS, RAF1, MAPK1/ERK2, MAPK3/ERK1), STAT family members, and PLCG1. SCF Protein, Rat (His) is the recombinant rat-derived SCF protein, expressed by E. coli with C-6*His labeled tag.
Description
Stem Cell Factor; SCF; KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2
Product Name Alternative
Stem Cell Factor; SCF; KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2
Accession Number
P21581-1
Tag
No-tag
Type
Cytokines and proteins
Applications
No tag
Purity
> 99 % as determined by SDS-PAGE
Activity
Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.1737 ng/mL.
Molecular Weight
18.47 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin : <1.0 EUper μg of the protein as determined by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Rat SCF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The Recombinant Rat SCF Protein consists of 170 amino acids and predicts a molecular mass of 18.47 KDa.
Formulation
Lyophilized from sterile 20mM Tris with 150 mM NaCl, pH 7.4.
Applications Notes
Rat
Other Product Names
Stem Cell Factor; SCF; KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Recombinant Rat SCF Protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprotkb/P21581/entry#sequences
AA Sequence
Amino acid sequence derived from Recombinant Rat SCF Protein P21581-1 (Q26-A189) was expressed.

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