Nucleolar Protein, 180kD (xNopp180) Antibody
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Catalog number
GEN607559
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Price
Please ask
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Size
5 ml
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Also known as
Nucleolar Protein, 180kD
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Other names
nucleolar protein; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
5F276
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Host organism
Mouse (Mus musculus)
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Source organism
Xenopus
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Species reactivity
Human (Homo sapiens), Xenopus; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Stains specifically the nucleolus and Cajal bodies in interphase cells. Recognizes 180kD polypeptide in Xenopus (xNopp180), homolog to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180. Species Crossreactivity: Xenopus laevis, Xenopus borealis, XLKE-A6 cells; negative with Pleurodelis, Triturus, Rana, chicken, rat, mouse.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Hybridoma culture supernatant
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Form Appearance
Supplied as a liquid in 0.09% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Nucleolar Protein, 180kD (xNopp180) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Alternative name
Anti-Nucleolar Protein, 180kD (xNopp180)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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