V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5) (FITC)

• Catalog number
  MBS606960
• Price
  Please ask
• Size
  0.1 mg

• Products_type
  Antibody
• Products_short_name
  [V5 Epitope Tag]
• Products_name_syn
  [Anti -V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5) (FITC)]
• Clonality
  Monoclonal
• Clone
  [9i128]
• Specificity
  Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
• Purity
  Affinity Purified Purified by Protein G affinity chromatography.
• Form
  Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 1% BSA. Labeled with FITC.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
• Tested_application
  Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Epitope   Tag   Pk-tag   Paramyxovirus   SV5  
• Short name
  V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5) (FITC)
• Technique
  FITC, Fluorescein, Tags
• Host
  Mouse
• Isotype
  IgG1
• Label
  tag
• Alternative name
  V5 Epitope detection labelled (Pk-detection labelled, Paramyxovirus, SV5) (fluorecein)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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