V-ATPase, epsilon subunit of tonoplast H+ATPase (affinty purified)

• Catalog number
  AS09 577A
• Price
  Please ask
• Size
  200 µg

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB)
• Connected products
  AS07 213 | V-ATPase | epsilon subunit of tonoplast H+ATPase rabbit antibodiesAS08 577 | V-ATPase | epsilon subunit of tonoplast H+ATPase goat antibodies, serum collection of antibodies to membrane transport systemmarker antibodies for plant cellular compartmentsPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1 : 1 000 with alkaline phosphatase or 1: 3000 with ECL (WB)
• Molecular weight expected аpparent
  26 | 31 kDa (Arabidopsis thaliana)
• Verified reactivity
  dicots including: Arabidopsis thaliana, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum, monocots including: Avena strigosa
• Possible reactivity
  Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp.,Phaseolus sp. ,Physcomitrella patens, Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, algae, Vitis vinifera bull frog, chicken, bovine, Drosophila melanogaster,human, mouse, rat
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
• References
  to be added when available. Antibody released in November 2009
• Scientific context
  Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
• Notes
  None
• Protein number
  Q39258
• TAIR number
  AT4G11150
• Hyperlink

• Gene target
  V-ATPase   epsilon   subunit   tonoplast   H+ATPase   affinty  
• Short name
  V-ATPase, epsilon subunit of tonoplast H+ATPase (affinty purified)
• Technique
  purified, purifications
• Alternative name
  V-ATPase, epsilon functionnal sequence on tonoplast HandATPase (affinty enriched)
• Alternative technique
  pure

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