Universal Protease (established for quantitative determination for HtrA-, MMP-, ADAMTS-Protease among other, using HtrA1 as standard) ELISA (Activity Assay) Kit

• Catalog number
  30 516 103
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• Size
  1 Microplate/40 determinations

• ELISA category
  ELISA (Activity Assay) Kit
• ELISA detection limits
  0.312 - 20 nM HtrA1
• ELISA sensitivity
  312 pM HtrA1
• ELISA s description
  The Universal Protease Activity ELISA measures activity of proteases like matrix metalloproteases (MMPs), A Disintegrin And Metalloproteinase with Thrombospondin Motifs (ADAMTS) and especially High temperature requirement factor A (HtrA). It consists of two modules: Protease module and the ELISA module.
• Research area interests
  Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
• Package form
  96 well microplate
• Antigen
  Protease
• Reacts with
  Protease
• Tested applications
  The casein based assay measures activity of proteases. It is used to screen and characterize inhibitors.
• Specificity
  Protease
• Crossreactivity
  N/A
• Preservative materials
  If applicable mentioned in the product data sheet
• Storage recommendations
  6 month
• Shipping and storage conditions
  dry Ice and ice pack
• Long term storage
  -20 °C and 4 °C
• Assay principle
  Proteolytic Reaction and Sandwich-ELISA
• Included in the standard package
  microplate, buffers, antibody conjugate,standards
• Not included in the standard package
  Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
• Samples to be analyzed
  biological fluids, cell culture
• PubMed citations
  See the datasheet
• Technical datasheet
  Contact us to receive the datasheet
• Representative figures
  more details see here read more
• Warnings
  For Research Use only.
• Other products
• Test
  biotez supplies other types of Assays as 1. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

• Gene target
  Universal   Protease   established   for   quantitative   determination   for   HtrA   MMP   ADAMTS-Protease   among   other   using   HtrA1   Activity   Kit  
• Gene symbol
  ADAMTS3, ADAMTS4, ADAMTS2, MMP24, MMP14, MMP28, MMP17, MMP15, MMP25, MMP16
• Short name
  Universal Protease (established for quantitative determination for HtrA-, MMP-, ADAMTS-Protease among other, using HtrA1 as standard) ELISA (Activity Assay) Kit
• Technique
  Standard, Assay, ELISA, assays, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. standards
• Host
  Assay, antigen assay ELISA kit 1 Kit (96 Wells)
• Alternative name
  Universal Protease (established to measure volumetric measurement to measure HtrA-, MMP-, ADAMTS-Protease among other, using HtrA serine peptidase 1 as reference) Enzyme-linked immunosorbent assay (Activity test) reagent
• Alternative technique
  standards, arrays, elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:219
• Gene
  ADAMTS3
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 3
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 3
• Synonyms
  • KIAA0366
  • ADAMTS-4
• GenBank acession
  • AB002364
• Locus
  4q13.3
• Discovery year
  1999-04-15
• Entrez gene record
  9508
• Pubmed identfication
  • 10094461
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000129912

Gene info

• Identity
  HGNC:220
• Gene
  ADAMTS4
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 4
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4
• Synonyms
  • KIAA0688
  • ADAMTS-2
  • ADMP-1
• GenBank acession
  • AB014588
• Locus
  1q23.3
• Discovery year
  1999-04-15
• Entrez gene record
  9507
• Pubmed identfication
  • 10094461
• RefSeq identity
  • NM_005099
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000034349

Gene info

• Identity
  HGNC:218
• Gene
  ADAMTS2
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 2
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2
• Synonyms
  • ADAMTS-3
  • hPCPNI
  • PCINP
  • ADAM-TS2
  • NPI
• Synonyms name
  • procollagen I N-proteinase
  • procollagen N-endopeptidase
• GenBank acession
  • AJ003125
• Locus
  5q35.3
• Discovery year
  1999-04-15
• Entrez gene record
  9509
• Pubmed identfication
  • 10094461
  • 15373769
• RefSeq identity
  • NM_014244
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000130915

Gene info

• Identity
  HGNC:7172
• Gene
  MMP24
• Long gene name
  matrix metallopeptidase 24
• Synonyms gene name
  • matrix metalloproteinase 24 (membrane-inserted)
  • matrix metallopeptidase 24 (membrane-inserted)
• Synonyms
  • MT5-MMP
• Synonyms name
  • membrane-type 5 matrix metalloproteinase
• GenBank acession
  • AF131284
• Locus
  20q11.22
• Discovery year
  1999-08-06
• Entrez gene record
  10893
• Pubmed identfication
  • 10363975
• RefSeq identity
  • NM_006690
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000032330

Gene info

• Identity
  HGNC:7160
• Gene
  MMP14
• Long gene name
  matrix metallopeptidase 14
• Synonyms gene name
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metallopeptidase 14 (membrane-inserted)
• Synonyms
  • MT1-MMP
• Synonyms name
  • membrane type 1 metalloprotease
  • membrane type 1-matrix metalloproteinase
• Locus
  14q11.2
• Discovery year
  1994-11-20
• Entrez gene record
  4323
• Pubmed identfication
  • 8015608
• RefSeq identity
  • NM_004995
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000028704

Gene info

• Identity
  HGNC:14366
• Gene
  MMP28
• Long gene name
  matrix metallopeptidase 28
• Synonyms gene name
  • matrix metalloproteinase 28
• Synonyms
  • MMP-25
  • MM28
  • EPILYSIN
  • MMP-28
• GenBank acession
  • AF315683
• Locus
  17q12
• Discovery year
  2001-01-09
• Entrez gene record
  79148
• Pubmed identfication
  • 11121398
  • 11255011
• RefSeq identity
  • NM_024302
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000188387

Gene info

• Identity
  HGNC:7163
• Gene
  MMP17
• Long gene name
  matrix metallopeptidase 17
• Synonyms gene name
  • matrix metalloproteinase 17 (membrane-inserted)
  • matrix metallopeptidase 17 (membrane-inserted)
• Synonyms
  • MT4-MMP
• GenBank acession
  • X89576
• Locus
  12q24.33
• Discovery year
  1996-11-13
• Entrez gene record
  4326
• Pubmed identfication
  • 9878265
• RefSeq identity
  • NM_016155
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000168050

Gene info

• Identity
  HGNC:7161
• Gene
  MMP15
• Long gene name
  matrix metallopeptidase 15
• Synonyms gene name
  • matrix metalloproteinase 15 (membrane-inserted)
  • matrix metallopeptidase 15 (membrane-inserted)
• Synonyms
  • MT2-MMP
  • MTMMP2
  • SMCP-2
• GenBank acession
  • Z48482
• Locus
  16q21
• Discovery year
  1996-11-13
• Entrez gene record
  4324
• Pubmed identfication
  • 9070935
  • 9119382
• RefSeq identity
  • NM_002428
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000133466

Gene info

• Identity
  HGNC:14246
• Gene
  MMP25
• Long gene name
  matrix metallopeptidase 25
• Synonyms gene
  • MMPL1
  • MMP20
• Synonyms gene name
  • matrix metalloproteinase 25
  • matrix metallopeptidase-like 1
• Synonyms
  • MT6-MMP
• GenBank acession
  • AF145442
• Locus
  16p13.3
• Discovery year
  2000-12-13
• Entrez gene record
  64386
• Pubmed identfication
  • 10628838
  • 10706098
• RefSeq identity
  • NM_022468
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000177475

Gene info

• Identity
  HGNC:7162
• Gene
  MMP16
• Long gene name
  matrix metallopeptidase 16
• Synonyms gene
  • C8orf57
• Synonyms gene name
  • matrix metalloproteinase 16 (membrane-inserted)
  • chromosome 8 open reading frame 57
  • matrix metallopeptidase 16 (membrane-inserted)
• Synonyms
  • MT3-MMP
  • DKFZp761D112
• GenBank acession
  • D85511
• Locus
  8q21.3
• Discovery year
  1996-11-13
• Entrez gene record
  4325
• Pubmed identfication
  • 7559440
• RefSeq identity
  • NM_005941
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000163769

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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