Ubiquitin-Fluorescein-labeled (FLR-Ub)

• Catalog number
  7552-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.
• Alternative_names
  UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.
• Description
  Substrate to determine the activity of ubiquitin conjugating enzymes
• Purity by SDS PAGE
  ≥95%
• Assay
  RP-HPLC
• Molecular Weight
  9.009 kDa
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  ≥ 8 mg/mL
• Appearance
  Liquid
• Physical form description
  ≥ 8 mg/mL in PBS
• Background Information
  Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Apart from the established role of Ub in protein degradation, it is implicated in cell signaling, DNA damage response, protein trafficking, cell-cycle progression, inflammation, immune response and regulation of apoptosis. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, BioVision’s fluorescein-labeled ubiquitin carries a single fluorescein molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  FITC labelling for flow cytometry of antibodies and peptides.

• Gene target
  Ubiquitin-Fluorescein   FLR-Ub  
• Gene symbol
  BLVRB
• Short name
  Ubiquitin-Fluorescein- (FLR-Ub)
• Alternative name
  Ubiquitin-Fluorescein-labeled (FLR-Ub)

Gene info

• Identity
  HGNC:1063
• Gene
  BLVRB
• Long gene name
  biliverdin reductase B
• Synonyms gene
  • FLR
• Synonyms gene name
  • Flavin reductase
  • biliverdin reductase B (flavin reductase (NADPH))
• Synonyms
  • SDR43U1
• Synonyms name
  • short chain dehydrogenase/reductase family 43U, member 1
  • (flavin reductase (NADPH))
• GenBank acession
  • D26308
• Locus
  19q13.2
• Discovery year
  1995-04-13
• Entrez gene record
  645
• Pubmed identfication
  • 7656592
  • 19027726
• RefSeq identity
  • NM_000713
• Classification
  • Short chain dehydrogenase/reductase superfamily
• VEGA ID
  OTTHUMG00000182593

MeSH Data

• Name
  Drug Labeling
• Concept
  Scope note: Use of written, printed, or graphic materials upon or accompanying a drug container or wrapper. It includes contents, indications, effects, dosages, routes, methods, frequency and duration of administration, warnings, hazards, contraindications, side effects, precautions, and other relevant information.
• Tree numbers
  • E05.916.310
• Qualifiers
  trends, ethics, history, classification, economics, instrumentation, methods, standards, legislation & jurisprudence, statistics & numerical data

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