TurboNuclease, Recombinant

Synonyms

TurboNuclease
Alternative_names

TurboNuclease
Description

A nonspecific endonuclease
Recombinant

Yes
Source

E. coli
Assay

SDS-PAGE
Unit Definition

>1.3x10⁶ units/mg. One unit of TurboNuclease converts 50 µg of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl₂.
Molecular Weight

27.0 kDa
Storage Temp

-20°C
Shipping

Gel Pack
Shelf Life

12 months
Concentration

250 U/µl
Appearance

Liquid
Physical form description

250 units/µl in a storage buffer of 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂ and 50% glycerol
Background Information

BioVision’s TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Benzonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. It is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet
Additional source

Recombinants or rec. proteins
Group

recombinants

Gene target

TurboNuclease
Short name

TurboNuclease, Recombinant
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

TurboNuclease, Rec.
Alternative technique

rec

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data