TMB Membrane Substrate

  • Catalog number
    42-TB07
  • Price
    Please ask
  • Size
    100 ml
  • Category
    Biological Reagents
  • Antibody Subtype
    Substrates
  • Area of research
    Miscellaneous
  • Grade Purity
    Highly pure
  • Form Buffer
    Provided as a liquid.
  • Storage
    Store at 4 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA
  • Usage Recommendations
    Optimal conditions to be determined by end user.  The product should be allowed to equilibrate to room temperature (25C) prior to use. For use, completely cover the membrane surface with substrate. The substrate system will react with sites on the membrane containing peroxidase producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. The reaction should be monitored and read before the background color becomes to intense which may result in diminished contrast between the positive and background staining. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
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  • Description
    Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 
  • Gene target
  • Short name
    TMB Substrate
  • Alternative name
    High sensitivity HRP membrane substrate (containing TMB)
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