THP-1 Cell Lysate

Description

Our lysate from THP-1 Cell is a high quality product which can readily be used in various applications such as Western Blot (WB) ELISA, EIA, IFA, etc. The THP-1 Cell Lysate will be an excellent control for these applications.
Storage

For shorter time periods the THP-1 Cell Lysate may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds which might cause change or loss of the desired properties.
Tips

Small amounts of the THP-1 Lysate may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
Additional description

For cells, cell lines and tissues in culture till half confluency.

Gene target

THP-1 Lysate
Gene symbol

GLI2, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
Short name

THP-1 Lysate
Alternative name

Lysate from THP-1 Cell
Tissue

cell, lysate
Virus

lysate

Identity

HGNC:4318
Gene

GLI2
Long gene name

GLI family zinc finger 2
Synonyms gene name
- GLI-Kruppel family member GLI2
- glioma-associated oncogene family zinc finger 2
Synonyms
- THP2
- HPE9
- THP1
Synonyms name
Locus

2q14.2
Discovery year

1989-05-25
Entrez gene record

2736
Pubmed identfication
- 2850480
- 9557682
RefSeq identity
- NM_005270
Classification
- Zinc fingers C2H2-type
VEGA ID

OTTHUMG00000153741

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081