Thioredoxin Reductase Activity Colorimetric Assay Kit

• Catalog number
  K763-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Absorbance (412 nm) • Species reactivity- Mammalian
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
• Features Benefits
  • Simple procedure; takes ~ 30-40 minutes • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. BioVision’s Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (λmax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Thioredoxin   Reductase   Activity   Kit  
• Short name
  Thioredoxin Reductase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Thioredoxin Reductase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products