TEV Protease Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K843-100
• Price
  Please ask
• Size
  100 assays

• Description
  Markets's first and only HTP adaptable assay kit to screen/study/characterize potential inhibitors of TEV Protease
• Summary
  Markets's first and only assay kit to fluorometrically (Ex/Em = 490/560 nm) screen/study/characterize potential inhibitors of TEV Protease. HTP adaptable.
• Detection Method
  Fluorescence (Ex/Em = 490/560 nm)
• Species Reactivity
  N/A
• Applications
  • Screening/studying/characterizing inhibitors of TEV Protease.
• Sample Type
  Chemical libraries, small molecules
• Features Benefits
  Simple, rapid assay HTP Adaptable Fluorometric Detection
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  TEV Protease (EC: 3.4.22.44) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). In the presence of a TEV Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of TEV Protease.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  TEV   Protease   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  TEV Protease Inhibitor Screening Kit (Fluorometric)
• Alternative name
  TEV Protease suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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