SuperBrite™ ECL Western Blot Substrate/Detection Kit

• Catalog number
  K824-50
• Price
  Please ask
• Size
  50 ml

• Description
  The most sensitive ECL Western blotting substrate kit on the market
• Summary
  The most sensitive ECL Western blotting substrate kit on the market. Application: Western Blotting, Dot Blotting.
• Detection Method
  Western blot. Dot blot
• Species Reactivity
  N/A
• Applications
  Western blot. Dot blot
• Sample Type
  pure proteins, cell/tissue extracts or lysates
• Features Benefits
  50 ml SuperBrite™ ECL Western Blotting solutions, sufficient for 30 min-gel blots or 2500 cm2 of membrane III. Advantages: • Minimal volumes required: Can be used for 30 mini-gel blots or 2500 cm2 of membrane. • Exceptional Sensitivity: Detects targets in the low picogram levels. • Multiple imaging options: Compatible with X-Ray, CCD and laser based imaging. • Ready to Use: No additional reconstitutions required. • Stable signal: Signals developed are stable. • Economical: Available at the most competitive rates.
• Storage Conditions
  +4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  BioVision’s SuperBrite™ ECL kit includes second-generation ECL substrates. They are highly sensitive, non-radioactive, and enhanced luminol-based chemiluminescent substrates for easy detection of horseradish peroxidase (HRP) on immunoblots. Blots can be repeatedly exposed to X-ray film or a CCD camera to obtain optimal results or stripped off the immune-detection reagents and re-probed.
• Data Sheet Link
• Test
  Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

• Gene target
  SuperBrite™   ECL   Substrate   Kit  
• Gene symbol
  CCL21
• Short name
  SuperBrite™ ECL Blot Substrate/ Kit
• Technique
  Western Blot, detection, Blot, western Blots
• Alternative name
  SuperBrite™ ECL Western Blot Substrate/quantification reagent
• Alternative technique
  western, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:10620
• Gene
  CCL21
• Long gene name
  C-C motif chemokine ligand 21
• Synonyms gene
  • SCYA21
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 21
  • chemokine (C-C motif) ligand 21
• Synonyms
  • SLC
  • exodus-2
  • TCA4
  • CKb9
  • 6Ckine
  • ECL
• Synonyms name
  • beta chemokine exodus-2
  • secondary lymphoid tissue chemokine
  • Efficient Chemoattractant for Lymphocytes
• GenBank acession
  • AB002409
• Locus
  9p13.3
• Discovery year
  1997-04-10
• Entrez gene record
  6366
• Pubmed identfication
  • 9235955
• RefSeq identity
  • NM_002989
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000019838

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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