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Category
Primary Antibodies
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Long description
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
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Antibody come from
Glycerol-3-phosphate dehydrogenase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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Clone
Polyclonal
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Antigen antibody binding interaction
Sheep anti Rabbit Glycerol-3-phosphate dehydrogenase antibody conjugated with Biotin Antibody
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Antibody is raised in
Sheep
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Antibody s reacts with
Rabbit
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Antibody s reacts with these species
Cross-reactivities anti enzymes of other sources may occur but have not been determined.
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Antibody s specificity
Biotin-conjugated IgG fraction of polyclonal Sheep antiSerum to glycerol-3-phosphate dehydrogenase from Rabbit muscle.
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Research interest
Enzymes, Veterinary
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
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Antibody s suited for
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/100 and 1/1,000.
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Storage
The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
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Relevant references
no information yet
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Protein number
P08507
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11, PIRC14, PIRC12
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Short name
anti Rabbit Glycerol-3-phosphate dehydrogenase antibody conjugated with
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Technique
Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates
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Host
Sheep, Rabbits
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Isotype
not specified
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Label
Biotin
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
ovine antibody to production species: rabbit Glycerol-3-phosphate dehydrogenase (antibody to-) coupled including biotinilated
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Alternative technique
antibodies, rabbit-anti
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