ROCK Activity Immunoblot test kit
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Catalog number
STA-415
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Price
Please ask
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Size
20 assays
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
Store active ROCK-II at -80ºC and all other test kit components at -20ºC. Avoid multiple freeze/thaw cycles.
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Technical file
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SDS or safty data sheet
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Type of reagent
Cell Signaling and Protein Biology
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Related techniques
Cell Signaling and Protein Biology, Kinase Assays, Rho Kinase (ROCK) Assays
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Use of the reagent
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our ROCK Activity Immunoblot test kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates.
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Cellular use
Safe non-radioactive Western blot assay format
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Living modified organisme in this reagent or LMO
Recombinant Protein from Baculovirus
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,0
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Gene target
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Short name
ROCK Activity Immunoblot test kit
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Alternative name
ROCK Activity Immunoblot test reagent
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Alternative technique
tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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