RFC1 Antibody

• Catalog number
  R30806
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  RFC1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the RFC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This RFC1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P35251
• Purity
  Antigen affinity
• Description
  Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
• Immunogen
  An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.
• Storage
  After reconstitution, the RFC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  RFC1  
• Gene symbol
  SLC19A1, RFC1
• Short name
  Anti-RFC1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to RFC1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10937
• Gene
  SLC19A1
• Long gene name
  solute carrier family 19 member 1
• Synonyms gene name
  • solute carrier family 19 (folate transporter), member 1
• Synonyms
  • FOLT
  • RFC1
• Synonyms name
  • reduced folate carrier 1
  • Folate transporter 1
• GenBank acession
  • U15939
• Locus
  21q22.3
• Discovery year
  1995-05-01
• Entrez gene record
  6573
• Pubmed identfication
  • 9570943
• Classification
  • Solute carriers
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000090397

Gene info

• Identity
  HGNC:9969
• Gene
  RFC1
• Long gene name
  replication factor C subunit 1
• Synonyms gene name
  • replication factor C (activator 1) 1 (145kD)
  • replication factor C (activator 1) 1, 145kDa
• Synonyms
  • A1
  • PO-GA
  • RFC140
  • MHCBFB
• GenBank acession
  • L23320
• Locus
  4p14
• Discovery year
  1994-10-14
• Entrez gene record
  5981
• Pubmed identfication
  • 8114700
• RefSeq identity
  • NM_002913
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000099363

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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