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Alternative name 1
Replication Fork Protein A, Clone: RPA34-19, Mab anti-Human; frozen/paraffin, IH/IP/WB
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Alternative name 2
Replication Fork Protein A, Clone: RPA34-19, Mab antibody-Human; frozen/paraffin, IH/IP/WB
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Alternative name 3
Replication Fork Protein A, Clone: RPA34-19, Mab antibody-Homo sapiens; frozen/paraffin, IH/IP/WB
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Alternative name 4
anti-Replication Fork Protein A Clone: RPA34-19 IP/
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Alternative name 5
Replication Fork Protein A Clone: RPA34-19 IP/
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
PIRC99, PIRC98, PIRC100, PIRC97, PIRC101
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Short name
Fork Protein A, Clone: RPA34-19, Mouse Monoclonal antibody- ; frozen/paraffin, IH/IP/
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Technique
Mouse monoclonal, Antibody, paraffin, frozen, Mouse, IP, WB, antibodies against human proteins, antibodies for, Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Western Blot on a membrane
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Human, Humans, Mouses
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Alternative name
Replication Fork Protein A, clonality: RPA34-19, Mouse monoclonal (antibody to-)-H. sapiens; -25°C frozen/paraffin, IH/IP/Western Blot
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Alternative technique
antibodies, murine
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Tissue
frozen
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