Recombinant Mouse HVEM / TNFRSF14 Protein (His & Fc tag)
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Catalog number
PKSM040930-100µg
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Price
Please ask
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Size
100µg
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Synonym
Atar;HveA;Hvem;Tnfrs14
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Activity
Measured by its binding ability in a functional ELISA.Immobilized mouse HVEM-Fch at 10 μg/mL (100 μl/well) can bind biotinylated mouse BTLA-Fc，The EC50 of biotinylated mouse BTLA-Fcis 64-96 ng/mL.
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Sequence
Met 1-Gln 206
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Fusion tag
C-His & Fc
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Accession
NP_849262.1
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 90 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
46.4 kDa
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AP Mol Mass
65 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFRSF14, TNFRSF14-AS1, TNFSF14
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Short name
Recombinant Mouse HVEM / TNFRSF14 Protein (His & Fc tag)
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Technique
Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
His
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse HVEM / tumor necrosis factor receptor superfamily, member 14 Protein (histidine & fragment c detection labelled)
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Alternative technique
rec, murine
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Alternative to gene target
tumor necrosis factor receptor superfamily, member 14, ATAR and CD270 and HVEA and HVEM and LIGHTR and TR2, TNFRSF14 and IDBG-86399 and ENSG00000157873 and 8764, ubiquitin protein ligase binding, Cell surfaces, Tnfrsf14 and IDBG-207237 and ENSMUSG00000042333 and 230979
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Gene info
Gene info
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Identity
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Gene
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Long gene name
TNFRSF14 antisense RNA 1
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Synonyms
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Locus
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Discovery year
2017-04-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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