Recombinant Human UCHL3 / UCH-L3 Protein (His tag)
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Catalog numberPKSH031053-20µg
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PricePlease ask
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Size20µg
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SynonymUCH-L3
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ActivityMeasured by the hydrolysis of UbiquitinAMC. The specific activity is >14,000 pmoles/min/μg.
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SequenceGlu 2-Ala 230
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Fusion tagN-His
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AccessionNP_005993.1
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Expressed HostE. coli
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity＞97 % as determined by SDS-PAGE
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EndotoxinPlease contact us for more information.
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass27 kDa
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AP Mol Mass26 kDa
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FormulationLyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0
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BackgroundUbiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolUCHL3, LMO7-AS1, RPL3, KLK9, UCHL1, MRPL38, CCR6, ADARB1
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Short nameRecombinant UCHL3 / UCH-L3 Protein (His tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens UCHL3 / UCH-L3 Protein (histidine detection labelled)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L3
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameLMO7 antisense RNA 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2014-03-25
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein L3
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- L ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekallikrein related peptidase 9
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Synonyms gene name
- kallikrein 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-03-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Kallikreins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L1
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Synonyms gene
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L38
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial ribosomal proteins
- Large subunit mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 6
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Synonyms gene
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Synonyms gene name
- chemokine (C-C motif) receptor 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameadenosine deaminase RNA specific B1
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Synonyms gene name
- adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Adenosine deaminases acting on RNA
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
