Recombinant Human Thyroid peroxidase / TPO Protein (257 Ser/Ala, 725 Pro/Thr, His tag)

• Catalog number
  PKSH030705-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  MSA;TDH2A;TPX
• Activity
  NA
• Sequence
  Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr
• Fusion tag
  C-His
• Accession
  P07202-1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  93.8 kDa
• AP Mol Mass
  90-100 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
• Background
  Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugate
  Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Thyroid   TPO   Protein   257   Ser/Ala   725   Pro/Thr   His   tag  
• Gene symbol
  TPO, THPO
• Short name
  Recombinant Thyroid / TPO Protein (257 Ser/Ala, 725 Pro/Thr, His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  peroxidase
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Thyroid peroxidase / TPO Protein (257 L-serine/alanine, 725 L-proline/L-threonine, histidine detection labelled)
• Alternative technique
  rec
• Tissue
  thyroid

Gene info

• Identity
  HGNC:12015
• Gene
  TPO
• Long gene name
  thyroid peroxidase
• Synonyms
  • TPX
• Locus
  2p25.3
• Discovery year
  1986-01-01
• Entrez gene record
  7173
• RefSeq identity
  • NM_000547
• Classification
  • Sushi domain containing
• VEGA ID
  OTTHUMG00000090271

Gene info

• Identity
  HGNC:11795
• Gene
  THPO
• Long gene name
  thrombopoietin
• Synonyms gene
  • MGDF
• Synonyms
  • TPO
  • MPLLG
• Synonyms name
  • prepro-thrombopoietin
  • megakaryocyte stimulating factor
  • myeloproliferative leukemia virus oncogene ligand
  • megakaryocyte growth and development factor
  • MPL ligand
  • megakaryocyte colony-stimulating factor
  • c-mpl ligand
  • thrombopoietin nirs variant 1
• Locus
  3q27.1
• Discovery year
  1994-11-04
• Entrez gene record
  7066
• Pubmed identfication
  • 8202154
• RefSeq identity
  • NM_000460
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000156745
• Locus Specific Databases
  • LRG_580

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products