Recombinant Human Semaphorin-3A/SEMA3A (C-6His)

• Catalog number
  CD65-10
• Price
  Please ask
• Size
  10 ug

• Description
  Recombinant Human Semaphorin 3A is produced by our Mammalian expression system and the target gene encoding Asn21-Val771 is expressed with a 6His tag at the C-terminus.
• Species reactivity
  Human
• Origin
  Human cells
• Peptide sequence
  NYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRATRAQDIRNGDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRTDQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRSVHHHHHH
• Estimated molecular weight
  87,3 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Ambient/Room Temperature
• Package form
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
• Storage conditions
  Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  Q14563
• Other related products
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Semaphorin-3A/SEMA3A   C-6His  
• Gene symbol
  IGHD3OR15-3A, H3-3A-DT, H3-3A, FAM102A, CCL20, SEMA3A
• Short name
  Recombinant Semaphorin-3A/SEMA3A (C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human Semaphorin 3A/SEMA3A(C-6His)
• Alternative technique
  rec
• Alternative to gene target
  sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, coll-1 and COLL1 and HH16 and Hsema-I and Hsema-III and SEMA1 and SEMAD and SEMAIII and SEMAL and SemD, SEMA3A and IDBG-24546 and ENSG00000075213 and 10371, chemorepellent activity, Extracellular, Sema3a and IDBG-135492 and ENSMUSG00000028883 and 20346, SEMA3A and IDBG-630984 and ENSBTAG00000018133 and 537777

Gene info

• Identity
  HGNC:5500
• Gene
  IGHD3OR15-3A
• Long gene name
  immunoglobulin heavy diversity 3/OR15-3A (non-functional)
• Synonyms gene name
  • immunoglobulin heavy diversity 3/OR15-3A
• Synonyms
  • IGHD3/OR15-3A
  • IGHD3OR153A
• GenBank acession
  • X55579
• Locus
  15q11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28331
• Pubmed identfication
  • 2114977
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000184598

Gene info

• Identity
  HGNC:55210
• Gene
  H3-3A-DT
• Long gene name
  H3-3A divergent transcript
• Locus
  1q42.12
• Discovery year
  2020-08-21
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4764
• Gene
  H3-3A
• Long gene name
  H3.3 histone A
• Synonyms gene
  • H3F3
  • H3F3A
• Synonyms gene name
  • H3 histone, family 3A
  • H3 histone family member 3A
• Synonyms
  • H3.3A
• GenBank acession
  • BC029405
• Locus
  1q42.12
• Discovery year
  1989-12-11
• Entrez gene record
  3020
• Pubmed identfication
  • 3031613
• RefSeq identity
  • NM_002107
• Classification
  • H3 histones
• VEGA ID
  OTTHUMG00000037507
• Locus Specific Databases
  • LRG_1410

Gene info

• Identity
  HGNC:31419
• Gene
  FAM102A
• Long gene name
  family with sequence similarity 102 member A
• Synonyms gene
  • C9orf132
• Synonyms gene name
  • chromosome 9 open reading frame 132
  • family with sequence similarity 102, member A
• Synonyms
  • Eeig1
  • bA203J24.7
  • SYM-3A
• Synonyms name
  • sym-3 homolog A (C. elegans)
  • early estrogen-induced gene 1
• Locus
  9q34.11
• Discovery year
  2005-03-22
• Entrez gene record
  399665
• Pubmed identfication
  • 23478294
• Classification
  • NT-C2 domain containing
• VEGA ID
  OTTHUMG00000020720

Gene info

• Identity
  HGNC:10619
• Gene
  CCL20
• Long gene name
  C-C motif chemokine ligand 20
• Synonyms gene
  • SCYA20
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • chemokine (C-C motif) ligand 20
• Synonyms
  • LARC
  • MIP-3a
  • exodus-1
  • ST38
  • CKb4
• GenBank acession
  • D86955
• Locus
  2q36.3
• Discovery year
  1997-02-10
• Entrez gene record
  6364
• Pubmed identfication
  • 9038201
  • 11352563
• RefSeq identity
  • NM_004591
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000133189

Gene info

• Identity
  HGNC:10723
• Gene
  SEMA3A
• Long gene name
  semaphorin 3A
• Synonyms gene
  • SEMAD
• Synonyms gene name
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• Synonyms
  • SEMA1
  • SemD
  • coll-1
  • Hsema-I
• Synonyms name
  • sema III
• GenBank acession
  • L26081
• Locus
  7q21.11
• Discovery year
  1999-06-25
• Entrez gene record
  10371
• Pubmed identfication
  • 8269517
  • 7748561
• RefSeq identity
  • NM_006080
• Classification
  • Immunoglobulin like domain containing
  • Semaphorins
• VEGA ID
  OTTHUMG00000023443

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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