Recombinant Human Prostate-Specific Antigen//PSA/KLK3 (Ala18-Pro261, C-6His)
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Catalog numberC365-50
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Price:
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Size50 ug
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DescriptionRecombinant Human Kallikrein 3 is produced by our Mammalian expression system and the target gene encoding Ala18-Pro261 is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginHuman cells
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Peptide sequenceAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANPVDHHHHHH
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Estimated molecular weight27,88 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionAmbient/Room Temperature
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Package formLyophilized from a 0.2 µm filtered solution of 20mM MES, 150mM NaCl, 2mM CaCl2, pH 6.0.
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Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP07288
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Additional descriptionAntigens are peptides or recombinant or native dependent on the production method.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolKLK3, KLKB1, PSAT1, NPEPPS
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Short nameRecombinant Prostate-Specific Antigen//PSA/KLK3 (Ala18-Pro261, C-6His)
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TechniqueRecombinant, antigen, antigenes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman Kallikrein 3/KLK3/PSA(Ala18-Pro261,C-6His)
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Alternative techniquerec, antigenes
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Tissueprostate
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Gene info
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Identity
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Gene
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Long gene namekallikrein related peptidase 3
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Synonyms gene
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Synonyms gene name
- kallikrein 3, (prostate specific antigen)
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Kallikreins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekallikrein B1
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Synonyms gene
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Synonyms gene name
- kallikrein B, plasma (Fletcher factor) 1
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Kallikreins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namephosphoserine aminotransferase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-05-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaminopeptidase puromycin sensitive
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M1 metallopeptidases
- Aminopeptidases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data