Recombinant Human Granzyme A/GZMA (C-6His)
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Catalog number
C355-50
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Price
Please ask
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Size
50 ug
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Description
Recombinant Human Granzyme A is produced by our Mammalian expression system and the target gene encoding Glu27-Val262 is expressed with a 6His tag at the C-terminus.
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Species reactivity
Human
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Origin
Human cells
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Peptide sequence
EKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLTEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAVVDHHHHHH
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Estimated molecular weight
27,11 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Ambient/Room Temperature
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Package form
Lyophilized from a 0.2 µm filtered solution of 20mM MES, 150mM NaCl, pH 5.5.
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Storage conditions
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P12544
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
GZMA
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Short name
Recombinant Granzyme A/GZMA (C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human Granzyme A/GZMA(C-6His)
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Alternative technique
rec
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Alternative to gene target
granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3), CTLA3 and HFSP, GZMA and IDBG-20850 and ENSG00000145649 and 3001, protein homodimerization activity, nuclei, Gzma and IDBG-183453 and ENSMUSG00000023132 and 14938, BT.58970 and IDBG-629315 and ENSBTAG00000021958 and 539093
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Gene info
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Identity
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Gene
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Long gene name
granzyme A
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Synonyms gene
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Synonyms gene name
- granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
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Synonyms name
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Locus
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Discovery year
1988-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granule associated serine proteases of immune defence
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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