Recombinant Human Arylsulfatase A / ARSA Protein (His tag)

• Catalog number
  PKSH031600-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  MLD
• Activity
  Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is >50 pmoles/min/μg.
• Sequence
  Met 1-Ala 507
• Fusion tag
  C-His
• Accession
  NP_000478.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞97 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  53 kDa
• AP Mol Mass
  53 kDa
• Formulation
  Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5
• Background
  Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Arylsulfatase   ARSA   Protein   His   tag  
• Gene symbol
  ARSA, GET3
• Short name
  Recombinant Arylsulfatase A / ARSA Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Arylsulfatase A / ARSA Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:713
• Gene
  ARSA
• Long gene name
  arylsulfatase A
• Synonyms name
  • metachromatic leucodystrophy
• GenBank acession
  • X52150
• Locus
  22q13.33
• Discovery year
  1986-01-01
• Entrez gene record
  410
• Pubmed identfication
  • 15772092
• RefSeq identity
  • NM_000487
• Classification
  • Sulfatases
• VEGA ID
  OTTHUMG00000150180

Gene info

• Identity
  HGNC:752
• Gene
  GET3
• Long gene name
  guided entry of tail-anchored proteins factor 3, ATPase
• Synonyms gene
  • ASNA1
• Synonyms gene name
  • arsA (bacterial) arsenite transporter, ATP-binding, homolog 1
• Synonyms
  • ARSA-I
  • TRC40
• Synonyms name
  • golgi to ER traffic 3 homolog (S. cerevisiae)
  • transmembrane domain recognition complex, 40kDa
• GenBank acession
  • U60276
• Locus
  19p13.13
• Discovery year
  1997-07-01
• Entrez gene record
  439
• Pubmed identfication
  • 8884272
  • 17382883
• RefSeq identity
  • NM_004317
• Classification
  • Guided entry of tail-anchored proteins complex subunits
  • GET4-GET5 transmembrane domain recognition complex subunits
• VEGA ID
  OTTHUMG00000180355

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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