Recombinant Human 14-3-3 Protein e/YWHAE (N-GST)
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Catalog numberCE07-1000
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PricePlease ask
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Size1 mg
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DescriptionRecombinant Human 14-3-3 Protein epsilon is produced by our E.coli expression system and the target gene encoding Met1-Gln255 is expressed with a GST tag at the N-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFHMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ
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Estimated molecular weight56 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionAmbient/Room Temperature
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Package formLyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
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Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP62258
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolYWHAQ, YWHAG, YWHAZ, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12
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Short nameRecombinant 14-3-3 Protein e/YWHAE (N-GST)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman 14-3-3 protein epsilon/YWHAE(N-GST)
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Alternative techniquerec
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Alternative to gene targettyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, 14-3-3E and HEL2 and KCIP-1 and MDCR and MDS, YWHAE and IDBG-14629 and ENSG00000108953 and 7531, phosphoprotein binding, Plasma membranes, Ywhae and IDBG-200597 and ENSMUSG00000020849 and 22627, YWHAE and IDBG-632682 and ENSBTAG00000005664 and 282125
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Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- protein, theta
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
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Synonyms gene
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Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 14
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 12
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data