Recombinant E. coli N-Acetyl-D-Glucosamine Kinase/NAGK (C-6His)

Description

Recombinant E.coli N-acetyl-D-Glucosamine Kinase is produced by our E.coli expression system and the target gene encoding Met1-Asp303 is expressed with a 6His tag at the C-terminus.
Species reactivity

E.coli
Origin

Escherichia coli
Peptide sequence

MNHKVHHHHHHMYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVCELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRLDNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRLPVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQGDEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPVARVPRIERARHGDAGGMRGAAFLHLTD
Estimated molecular weight

34,5 kDa
Protein purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level

Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Shipping condition

Dry ice/ice packs
Package form

Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl,2mM DTT,20% Glycerol, pH 8.0.
Storage conditions

Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Reconstitution conditions

See included datasheet or contact us for more information.
UniProt number

P75959
Other related products
Additional description

N terminal acetylation or CH3CO as epigenetic regulation of Recombinant N- -D-Glucosamine Kinase/NAGK (C-6His) by NATs.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

D-Glucosamine Kinase/NAGK C-6His
Gene symbol

NAGK
Short name

Recombinant N- -D-Glucosamine Kinase/NAGK (C-6His)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Species

E. coli, E. coli
Alternative name

Recombinant E.coli NAGK(C-6His)
Alternative technique

rec, escherichia

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data