Mouse PDGF-B (Platelet Derived Growth Factor Subunit B) ELISA Kit
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Catalog numberEKE61750
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PricePlease ask
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Size96 Tests
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Estimated Turnaround Time6-11 business days
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Target NameNA
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Target s alterntive namePDGF-B
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Species ReactivityMouse
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Sample TypeSerum, Plasma, Biological Fluids
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Detection Range31.25-2000 pg/mL
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Sensitivity18.75 pg/mL
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PrecisionNA
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Detection MethodColorimetric
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Assay Time4.5 hours
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ApplicationFor research use only. Not for diagnostic procedures.
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Assay TypeSandwich
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Shipping ConditionsIce packs
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Storage TemperatureShort term: 4°C; Long term: see manual.
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Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf lifeUse Mouse PDGF-B (Platelet Derived Growth Factor Subunit B) ELISA Kit before 6 months
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SpecificityNA
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Cross ActivityNo significant cross-reactivity or interference was observed.
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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFB . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PDGFB. The concentration of Mouse PDGFB in samples can be calculated by comparing the OD of the samples to the standard curve.
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StabilityNA
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Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
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Research AreaCancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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DescriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
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Latin nameMus musculus
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Gene target
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Gene symbolPDGFB, PDGFA, SNRPB, ORM2, ERAL1, OR1B1, SPIB, EXPH5, RELB, HLA-B
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Short nameMouse PDGF-B (Platelet Derived Growth Factor Subunit B) ELISA Kit
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TechniqueELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Hostmouse, antigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesMouse, Mouses
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Alternative nameMouse PDGF-B (Platelet Derived Growth Factor functionnal sequence B) Enzyme-linked immunosorbent assay reagent
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Alternative techniquemurine, elisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameplatelet derived growth factor subunit B
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Synonyms gene
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Synonyms gene name
- platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
- platelet-derived growth factor beta polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameplatelet derived growth factor subunit A
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Synonyms gene name
- platelet-derived growth factor alpha polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nuclear ribonucleoprotein polypeptides B and B1
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Synonyms gene
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Synonyms
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Locus
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Discovery year1988-11-28
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Entrez gene record
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Pubmed identfication
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Classification
- Small nucleolar RNA protein coding host genes
- Sm spliceosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameorosomucoid 2
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-03-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lipocalins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEra like 12S mitochondrial rRNA chaperone 1
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Synonyms gene name
- Era (E. coli G-protein homolog)-like 1
- Era G-protein-like 1 (E. coli)
- Era-like 12S mitochondrial rRNA chaperone 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-19
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily B member 1
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Synonyms gene name
- olfactory receptor, family 1, subfamily B, member 1 (gene/pseudogene)
- olfactory receptor family 1 subfamily B member 1 (gene/pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-09
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Entrez gene record
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RefSeq identity
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Classification
- Olfactory receptors, family 1
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSpi-B transcription factor
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Synonyms gene name
- Spi-B transcription factor (Spi-1/PU.1 related)
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Synonyms
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Locus
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Discovery year1994-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameexophilin 5
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Synonyms
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Synonyms name
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Locus
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Discovery year2005-10-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRELB proto-oncogene, NF-kB subunit
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Synonyms gene name
- v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-10-02
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Entrez gene record
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Pubmed identfication
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Classification
- IPT domain containing
- NF-kappa B complex subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, B
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Synonyms gene
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Synonyms gene name
- ankylosing spondylitis
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
