-
Category
Serums
-
Long description
This standard is prepared by pooling serum obtained from a large number rats with the following assigned values: IgG1--1.68 mg/ml. IgG2a--3.36 mg/ml. IgG2b--7.66 mg/ml. IgG2c--0.50 mg/ml. IgA--2.43 mg/ml. IgM--1.31 mg/ml. The determination of the concentration of each of the four subclasses was carried out by single radial immunodiffusion (RID, Mancini) in agar gel Nordic Nr. 2 using subclass-specific polyclonal antisera and purified rat immunoglobulins as primary standards. Rat standard serum NOR-03 is intended for use as a calibrator to prepare standard curves in quantitative determinations of the immunoglobulins IgA and IgM and IgG subclasses.
-
Antibody come from
n/a
-
Other description
Delipidated, lyophilized serum. No preservative added.
-
Clone
not specified
-
Antigen antibody binding interaction
Rat Ig and IgG subclasses standard Serum
-
Antibody is raised in
see techfile
-
Antibody s reacts with
Rat
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
Rat standard Serum with assigned values for IgA, IgM and IgG subclasses.
-
Research interest
Immunology
-
Application
not tested yet / to be tested
-
Antibody s suited for
The product is shipped lyophilized at ambient temperature. The lyophilized content is reconstituted by adding 0.5 ml sterile water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted serum may be divided into small aliquots, frozen at or below -20°C. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS) containing 0.05% Tween and 1% (w/v) bovine serum albumin, should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
-
Storage
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
-
Relevant references
no information yet
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats. Serum should be stored at +5°C
-
Latin name
Rattus norvegicus
-
Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
-
Group
Sera
