Rabbit anti-rat Thy-1/CD90

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• Catalog number
  MUB2058
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• Size
  0,1mL

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• Category
  Primary Antibodies
• Long description
  Thymocyte-1 antigen (Thy-1), also known as CD90, is a glycophosphatidylinositol (GPI)-anchored cell surface glycoprotein that is a member of the immunoglobulin superfamily. Expression of Thy-1, originally identified as a marker of thymocyte differentiation in mice, has been found to vary between species. In rats, unlike mice, Thy-1 is expressed on bone marrow cells but not on peripheral T-cells. In both mice and rats, Thy-1 is abundantly expressed on thymocytes and neuronal cells and to a lesser extent on fibroblasts, myofibroblasts, vascular endothelial cells, mammary glands and other non-lymphoid tissues. In many tissues the level of Thy-1 expression changes during cell differentiation. The exact function of Thy-1 has not been fully determined but studies suggest that Thy-1 may be an important regulator of cell-cell and cell-matrix interactions due to its involvement in a range of biological processes including cell growth and differentiation, cell adhesion and migration, apoptotic signalling and tumour suppression.
• Antibody come from
  Thy-1 antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with purified Thy-1 derived from rat brain.
• Other description
  Each vial contains 100µl polyclonal antiserum containing 0.02% sodium azide.
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Rabbit anti-rat Thy-1/CD90 Antibody
• Antibody is raised in
  Rabbit
• Antibody s reacts with
  Rat, Mouse
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  Thy-1 antiserum is directed against rat thymocyte-1 antigen (Thy-1) and also reacts with mouse Thy-1.
• Research interest
  CD Marker, Cell adhesion, Neurobiology
• Application
  Immunocytochemistry, Immunohistochemistry (frozen)
• Antibody s suited for
  Thy-1 is suitable for the detection of rat and mouse Thy-1 by immunohistochemical staining of frozen tissue sections. After drying, the sections can be either left unfixed or fixed with acetone at 4°C for 4 min or 95% ethanol at 4°C for 15 min or 4% formaldehyde in phosphate buffered saline pH 7.4 at 20°C for 15 min. Also isolated cells can be immunocytochemically stained by this antiserum. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range 1:50 – 1:100.
• Storage
  Store at 4oC, or in small aliquots at -20oC.
• Relevant references
  1. Barclay AN, Letarte-Muirhead M, Williams AF. (1975) Purification of the Thy-1 molecule from rat brain. Biochem J. 151:699-706. 2. Letarte-Muirhead M, Barclay AN, Williams AF. (1975) Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. Biochem J. 151:685-97. 3. Barclay AN, Hydén H. (1978) Localization of the Thy-1 antigen in rat brain and spinal cord by immunofluorescence. J Neurochem. 31:1375-91. 4. Barclay AN. (1979) Localization of the Thy-1 antigen in the cerebellar cortex of rat brain by immunofluorescence during postnatal development. J Neurochem. 32:1249-57. 5. Barclay AN, Hydén H. (1979) Localization of the Thy-1 antigen by immunofluorescence on neurons isolated from rat brain. J Neurochem. 32:1583-86.
• Protein number
  UniProt: P01830 (Rat), P01831 (Mouse)
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Oryctolagus cuniculus, Rattus norvegicus

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• Gene target
  Thy-1/CD90  
• Gene symbol
  THY1
• Short name
  Rabbit anti- Thy-1/CD90
• Technique
  Rabbit, anti-, anti, antibody to, antibodies
• Host
  Rabbit, Rabbits
• Isotype
  not specified
• Label
  unlabeled
• Species
  Rat, Rats
• Alternative name
  production species: rabbit Antibody torat Thy-1/CD90
• Alternative technique
  rabbit-anti, antibodies

Gene info

• Identity
  HGNC:11801
• Gene
  THY1
• Long gene name
  Thy-1 cell surface antigen
• Synonyms
  • CD90
• GenBank acession
  • M11749
• Locus
  11q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  7070
• Pubmed identfication
  • 2864690
• RefSeq identity
  • NM_006288
• Classification
  • Immunoglobulin like domain containing
  • Ig-like cell adhesion molecule family
  • CD molecules
• VEGA ID
  OTTHUMG00000166202

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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