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Immunogen
Full length recombinant protein HSP17.7 produced in E.coli and purified by conventional methods (no affinity tag)
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Antibody s target
Plant HSP17.7
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Antibody s full description
Rabbit Anti-Plant HSP17.7 (Plant) Polyclonal
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Heat shock protein 17.7 Antibody, 17.7 kDa class II heat shock protein antibody, 17.7 kDa heat shock protein Antibody, AtHSP17.7 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Plant, Arabidopsis thaliana
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Rabbit Antiserum
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Recommended buffer for storage
Lyophilized rabbit Antiserum. For reconstitution add 200 µl of sterile water.
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 17.7kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1.0 µl/ml of SPC-309 was sufficient for detection of HSP17.7 in 15 µg of heat shocked (38° C for 2 hrs) Arabidopsis thaliana lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
HSP17.7 belongs to the class II family of small heat shock proteins. Small HSPs are induced when plant cells are stressed by increased temperature (1). How small HSPs protect living cells is not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small HSPs are also produced late in the seed maturation process.
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Bibliography
1. Malik M.K., Slovin J.P., Hwang C.H., Zimmerman J.L. (1999) Plant J. 20(1): 89-99.
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Released date
1/Jan/2009
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NCBI number
NP_196764.1
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Gene number
831076
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Protein number
O81822
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Arabidopsis tissue lysates showing detection of HSP17.7 protein using Rabbit Anti-HSP17.7 Polyclonal Antibody (SPC-309). Primary Antibody: Rabbit Anti-HSP17.7 Polyclonal Antibody (SPC-309) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP-conjugated Goat Anti-Rabbit at 1:10000. 15ug of total protein from (left to right): heat-shocked Arabidopsis thalina, Arabidopsis thaliana control plants, 1,2, 5 and 10ng of recombinant purified Hsp17.7. Band at ~38 kDa represents an SDS resistant dimer. Rabbit Anti-HSP17.7 Antibody used in Western blot (WB) on Arabidopsis tissue lysates (SPC-309)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
