-
Immunogen
Synthesized peptide derived from human osteopontin .
-
Antibody s target
Human Osteopontin
-
Antibody s full description
Rabbit Anti-Human Osteopontin Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
BNSP Antibody, Bone sialoprotein 1 Antibody, BSP I Antibody, BSPI Antibody, Early T lymphocyte activation 1 Antibody, ETA 1 Antibody, ETA1 Antibody, MGC110940 Antibody, Nephropontin Antibody, OPN Antibody, Osteopontin Antibody, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein Antibody, OSTP_HUMAN Antibody, PSEC0156 Antibody, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) Antibody, Secreted phosphoprotein 1 Antibody, SPP 1 Antibody, SPP-1 Antibody, SPP1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody
-
Verified applications
WB, ELISA
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat
-
Antibody s recommended dilutions for use
WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects ~35kDa, endogenous levels of total osteopontin protein.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-1305 was sufficient for detection of Osteopontin in 10 µg of LOVO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Secreted
-
Tissue specificity
Bone. Found in plasma.
-
Scientific context
Osteopontin is a single-chain polypeptide. The gene has 7 exons, spans 5 kilobases in length and in humans it is located on the long arm of chromosome 4 region 22 (4q1322.1). The protein is composed of ~300 amino acids residues and has ~30 carbohydrate residues attached including 10 sialic acid residues, which are attached to the protein during post-translational modification in the Golgi apparatus. The protein is rich in acidic residues: 30-36% are either aspartic or glutamic acid. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling.
-
Bibliography
1. Wang KX and Denhardt DT (2008) Cytokine Growth Factor Rev. 19 (5-6): 333–45. 2. Rangaswami H. et al. (2006) Trends Cell Biol. 16 (2): 79–87. 3. Young MF. et al. (1990) Genomics. 7 (1): 491–502.
-
Released date
6/Sep/2016
-
NCBI number
NP_000573.1
-
Gene number
6696
-
Protein number
P10451
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human LOVO cell lysates showing detection of ~35kDa Osteopontin protein using Rabbit Anti-Osteopontin Polyclonal Antibody (SPC-1305). Lane 1: Human LOVO cells. Lane 2: Human LOVO cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Osteopontin Polyclonal Antibody (SPC-1305) at 1:1000. Predicted/Observed Size: ~35kDa. Other Band(s): ~60kDa. Rabbit Anti-Osteopontin Antibody used in Western blot (WB) on LOVO cell lysates (SPC-1305)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugate
Peridinin chlorophyll (PerCP)
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
SPP1
-
Short name
Rabbit Anti- Osteopontin Polyclonal: PerCP
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- Osteopontin Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
IgG
-
Label
PerCP
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman Osteopontin polyclonal: PerCP
-
Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
-
