Rabbit Anti-Human HSF2 Polyclonal: Streptavidin
-
Catalog numberSPC-712D-STR
-
PricePlease ask
-
Size100 µg
-
-
ImmunogenSynthetic peptide from the mid-protein of Human HSF2
-
Antibody s targetHuman HSF2
-
Antibody s full descriptionRabbit Anti-Human HSF2 Polyclonal
-
Primary research fieldsCell Signaling, Epigenetics and Nuclear Signalling, Transcription
-
Antibody s categoryPolyclonal Antibodies
-
Antibody s other nameHeat shock factor protein 2 Antibody, Heat shock transcription factor 2 Antibody, HSTF 2 Antibody, HSTF2 Antibody, HSF 2 Antibody, HSF2 Antibody, HSF2_HUMAN Antibody
-
Verified applicationsWB
-
Raised inRabbit
-
Antibody s reactivityRat
-
Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified fromPeptide Affinity Purified
-
Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~90 kDa.
-
Storage recommendations-20°C
-
Shipping recommendationsBlue Ice or 4°C
-
Certificate of analysisA 1:1000 dilution of SPC-712 was sufficient for detection of HSF2 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasm, Nucleus
-
Scientific contextSee included datasheet or contact our support service.
-
BibliographyRefer to PubMed
-
Released date25/Oct/2016
-
NCBI numberNP_001129036.1
-
Gene number3298
-
Protein numberQ03933
-
PubMed numberRefer to PubMed
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
-
Representative figure legendWestern blot analysis of Rat Liver cell lysates showing detection of ~60.3 kDa HSF2 protein using Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSF2 Polyclonal Antibody (SPC-712) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~60.3 kDa. Rabbit Anti-HSF2 Antibody used in Western blot (WB) on Liver cell lysates (SPC-712)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilitIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Gene symbolHSF2
-
Short nameRabbit Anti- HSF2 Polyclonal: Streptavidin
-
Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
HostRabbit, Rabbits
-
IsotypeImmunoglobulin
-
LabelStreptavidin
-
SpeciesHuman, Humans
-
Alternative nameproduction species: rabbit Antibody toHuman HSF2 polyclonal: biotinilated-binding streptavidin
-
Alternative techniquepolyclonals, rabbit-anti, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameheat shock transcription factor 2
-
GenBank acession
-
Locus
-
Discovery year1994-09-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data