-
Immunogen
Synthetic peptide from the mid to C-terminal of Human IFT88 (aa. 674-686)
-
Antibody s target
Human IFT88
-
Antibody s full description
Rabbit Anti-Human IFT88 Polyclonal
-
Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, DNA/RNA, Translation
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
D13S1056E Antibody, TPR repeat protein 10 Antibody, Tetratricopeptide repeat domain 10 Antibody, Ift88 Antibody, Recessive polycystic kidney disease protein Tg737 homolog Antibody, IFT88_HUMAN Antibody, Polaris homolog Antibody, Probe hTg737 (polycystic kidney disease, autosomal recessive) Antibody, TG737 Antibody, TTC10 Antibody, DAF19 Antibody, IFT88 Antibody, hTg737 Antibody, Intraflagellar transport 88 homolog Antibody, MGC26259 Antibody, Tetratricopeptide repeat protein 10 Antibody, Intraflagellar transport protein 88 homolog Antibody, RP11-172H24.2 Antibody
-
Verified applications
WB
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse
-
Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~94 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-679 was sufficient for detection of IFT88 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Centrosome, Centriole, Cytoplasm, Cytoskeleton, Cilium Basal Body
-
Tissue specificity
Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
-
Scientific context
See included datasheet or contact our support service.
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_001305420.1
-
Gene number
8100
-
Protein number
Q13099
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human A549 cell lysates showing detection of 94.3 kDa IFT88 protein using Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 94.3 kDa. Other Band(s): 45 kDa. Rabbit Anti-IFT88 Antibody used in Western blot (WB) on A549 cell lysates (SPC-679)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
IFT88
-
Short name
Rabbit Anti- IFT88 Polyclonal: Streptavidin
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
Streptavidin
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman intraflagellar transport 88 homolog (Chlamydomonas) polyclonal: biotinilated-binding streptavidin
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
intraflagellar transport 88 homolog (Chlamydomonas), D13S1056E and DAF19 and hTg737 and TG737 and TTC10, IFT88 and IDBG-13468 and ENSG00000032742 and 8100, protein binding, multiple, Ift88 and IDBG-168502 and ENSMUSG00000040040 and 21821, BT.36147 and IDBG-631299 and ENSBTAG00000014286 and 514177
-
