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Immunogen
Synthesized unphosphorylated peptide derived from human Doublecortin around the phosphorylation site of serine 376 (P-T-SP-P-G).
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Antibody s target
Human Doublecortin
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Antibody s full description
Rabbit Anti-Human Doublecortin Polyclonal
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Primary research fields
Neuroscience, Cell Structure
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
DBCN Antibody, Dbct Antibody, DC Antibody, DCX Antibody, DCX_HUMAN Antibody, Doublecortex Antibody, Doublin Antibody, FLJ51296 Antibody, Lis X Antibody, Lis-X Antibody, Lissencephalin X Antibody, Lissencephalin-X Antibody, Lissencephaly X linked Antibody, Lissencephaly X linked doublecortin Antibody, LISX Antibody, Neuronal migration protein doublecortin Antibody, OTTHUMP00000023859 Antibody, OTTHUMP00000023860 Antibody, OTTHUMP00000216315 Antibody, OTTHUMP00000216316 Antibody, SCLH Antibody, XLIS Antibody
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Verified applications
WB, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~45kDa, endogenous levels of total Doublecortin protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1294 was sufficient for detection of Doublecortin in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytosol, Cytoskeleton, Cell projection
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Tissue specificity
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
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Scientific context
Neuronal migration protein doublecortin is a protein that in humans is encoded by the DCX gene, also known as doublin or lissencephalin-X. Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. It seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway.
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Bibliography
1. Oomen C.A. et al. (2009) PLoS ONE 4 (1): e3675. 2. Brown J.P. et al. (2003) J. Comp. Neurol. 467 (1): 1–10. 3. Couillard-Despres S. et al. (2005) Eur. J. Neurosci. 21 (1): 1–14.
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Released date
6/Sep/2016
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NCBI number
NP_000546.2
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Gene number
1641
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Protein number
O43602
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human Jurkat cell lysates showing detection of ~40-45kDa Doublecortin protein using Rabbit Anti-Doublecortin Polyclonal Antibody (SPC-1294). Lane 1: Human Jurkat. Lane 2: Human Jurkat treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Doublecortin Polyclonal Antibody (SPC-1294) at 1:1000. Predicted/Observed Size: ~40-45kDa. Rabbit Anti-Doublecortin Antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1294)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
