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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr439 and Thr440 of human Abl2 (AA3437-443)
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Antibody s target
Human Abl2 (pTyr439 + pThr440)
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Antibody s full description
Rabbit Anti-Human Abl2 (pTyr439 + pThr440) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Abelson tyrosine protein kinase 2 Antibody, ABL2 Antibody, ARG Antibody, Tyrosine kinase ARG Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 128.343 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-906 was sufficient for detection of Abl2 (pTyr439 + pThr440) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytoskeleton
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Tissue specificity
Widely expressed
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Scientific context
ABL2 (Arg) is a widely expressed, 128 kDa, non-receptor protein-tyrosine kinases that is structurally related to Abl1. It features SH3 and SH2 domains, a protein- tyrosine kinase domain, and a nuclear localization domain. It shares many functions with Abl1, which includes cytoskeleton remodelling through phosphorylation of enzymes responsible for cytoskeleton rearrangements for signalling, adhesion, and movement as well as microtuble subunits, TUBA1 and TUBB. It also contains F-actin binding region to bundle and regulate actin cytoskeletal structure. It possesses autocatalytic activity and the ability to phosphorylate its inhibitor ABI1 to regulate itself.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_009298
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Gene number
27
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Protein number
P42684
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugate
Peridinin chlorophyll (PerCP)
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit Anti- Abl2 (pTyr439 + pThr440) Polyclonal: PerCP
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Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- Abl2 (pTyr439 + pThr440) Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
PerCP
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman v-abl Abelson murine leukemia viral oncogene homolog 2 (pTyr439 + pThr440) polyclonal: PerCP
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Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
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Alternative to gene target
v-abl Abelson murine leukemia viral oncogene homolog 2, ABLL and ARG, ABL2 and IDBG-105099 and ENSG00000143322 and 27, transferase activity, Cytoplasm, Abl2 and IDBG-200390 and ENSMUSG00000026596 and 11352, BT.62162 and IDBG-636736 and ENSBTAG00000015026 and 511845
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