R Spondin 1 human recombinant
R Spondin 1 human recombinant is available 2 times from Biovision labs
- BackgroundR-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Recombinant human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS PAGE analysis under reducing conditions.
- Molecular weight40.0 kDa
- SynonymRoof plate-specific Spondin, Rspo1
- Other nameRoof plate-specific Spondin, Rspo1
- NCBI gene number284654
- NCBI geneRSPO1
- Gene sourceHuman
- NCBI numberQ2MKA7
- SourceCHO Cells
- Tested applicationsSDS-PAGE, HPLC
- Level of endotoxin< 0.1 ng/μg of protein (
- Tested activityR-spondin-1 enhances BMP-2 mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml.
- Biological activityR-spondin-1 enhances BMP-2 mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml.
- Binding abilityN/A
- Storage condition-20°C
- Shipping underGel Pack
- Physical appearanceLyophilized powder
- Physical propertiesSterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.
- How to reconstituteCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
- Aa sequenceSRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
- Before useCentrifuge the vial prior to opening.
- NotesFor Research Use Only! Not to be used in humans
- PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
- Additional sourceRecombinants or rec. proteins
- Gene target
- Short nameR-Spondin-1, recombinant
- Alternative nameR-Spondin-1, H. sapiens recombinant
- Alternative techniquerec
7189-10 | R-Spondin-1, human recombinantsize: 10 μg | 255.86 USD
- Catalog number7189-10
- Price255.86 USD
- Size10 μg
1. Gene info
- Long gene nameR-spondin 1
- Synonyms gene name
- R-spondin homolog (Xenopus laevis)
- GenBank acession
- Discovery year2005-08-08
- Entrez gene record
- RefSeq identity
- Endogenous ligands
- Havana BLAST/BLAT
- ConceptScope note:The initial culturing of cells derived directly from fresh TISSUES.
- Tree numbers
- Qualifiersclassification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
- PubMedR-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/beta-catenin signaling in the uncommitted mesenchymal C2C12 cells.We highlight the cooperation of WNT4, RSPO1 and FOXL2 within a regulatory network and the need for further research to better understand their role in defining and maintaining ovarian identity.identified a gene, R-spondin1, with potent and specific proliferative effects on intestinal crypt cellsMice treated with R-spondin1 showed increased intestinal epithelial healing, providing a protective effect against chemotherapy-induced intestinal mucositis.Mutational analysis of the RSPO1 gene in a 46,XX woman with true hermaphroditism, palmoplantar keratoderma, congenital bilateral corneal opacities, onychodystrophy, and hearing impairment, is reported.study provides new mechanistic insights into the regulation of Wnt receptor turnover, and reveals ZNRF3 as a tractable target for therapeutic explorationIn conclusion, present study highlights the role of Rspo 1 in bone remodeling where it activates Wnt signaling to induce differentiation, as shown in human as well murine in vitro osteoblast cell models.
- Gene ontology - Biological process
- Gene ontology - Cellular component
- Gene ontology - Molecular function
- Entrez Gene
7189-50 | R-Spondin-1, human recombinantsize: 50 μg | 830.32 USD