Protein A Sepharose

Protein A Sepharose is available 10 times from Biovision labs

6501-5 | Protein A Sepharosesize: 5 ml | 455.64 USD

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6501-25 | Protein A Sepharosesize: 25 ml | 1076.45 USD

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6501-1 | Protein A Sepharosesize: 1 ml | 156.06 USD

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6501-100 | Protein A Sepharosesize: 100 ml | 2785.1 USD

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6501-1 | Protein A Sepharosesize: 2X1 ml | 364.51 USD

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M1300-1 | Protein A Sepharose (Endotoxin-free)size: 1 ml | 392.99 USD

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M1300-5 | Protein A Sepharose (Endotoxin-free)size: 5 ml | 609.42 USD

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M1300-25 | Protein A Sepharose (Endotoxin-free)size: 25 ml | 1987.73 USD

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6508-5 | Protein A Sepharose Columnsize: 5 ml | 689.16 USD

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6508-1 | Protein A Sepharose Columnsize: 1 ml | 336.03 USD

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  • Packaging
    EA
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Category
    Protein A
  • Highlights
    •CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H2O. - HIGH BINDING CAPACITY: Binding of IgG ≥16 mg human or rabbit IgG/ml Protein A-Sepharose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 2.07 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
  • Description
    Protein A-Sepharose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose beads. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Sepharose is ≥ 16 mg human or rabbit IgG per ml of wet beads. Protein A-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
  • Storage condition
    +4°C
  • Shipping condition
    gel pack
  • Maximum time for storage
    12 months
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