Pyruvate Kinase Antibody / PKM2

• Catalog number
  R31966
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Pyruvate Kinase / PKM2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the PKM2 antibody should be determined by the researcher.
• Intented use
  This PKM2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P14618
• Purity
  Antigen affinity
• Description
  PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
• Immunogen
  Amino acids KDPVQEAWAEDVDLRVNFAMNVGKAR of human PKM2 were used as the immunogen for the PKM2 antibody.
• Storage
  After reconstitution, the PKM2 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Pyruvate   Kinase   PKM2  
• Gene symbol
  PKM
• Short name
  Anti-Pyruvate Kinase / PKM2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Pyruvate Kinase / PKM2
• Alternative technique
  antibodies
• Alternative to gene target
  pyruvate kinase, muscle, CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, poly(A) RNA binding, nuclei, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571

Gene info

• Identity
  HGNC:9021
• Gene
  PKM
• Long gene name
  pyruvate kinase M1/2
• Synonyms gene
  • PKM2
• Synonyms gene name
  • pyruvate kinase, muscle
• Synonyms
  • THBP1
  • OIP3
  • PK3
• GenBank acession
  • M23725
• Locus
  15q23
• Discovery year
  2001-06-22
• Entrez gene record
  5315
• Pubmed identfication
  • 2040271
• VEGA ID
  OTTHUMG00000172709

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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