Rabbit Anti Human PIWIL4 Antibody-Non-conjugated
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Catalog numberCSB-PA767195LA01HU_100ug
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PricePlease ask
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Size100ug
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Long nameRabbit Antibody against Human PIWIL4, Non-conjugated
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Synonym namePIWIL4 Human Antibody
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Species reactivityHuman
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Tested applications recommended dilutionsELISA,WB,IF;Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200
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ConjugationNon-conjugated
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Antibody isotypeIgG
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UniProt IDQ7Z3Z4
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant Human Piwi-like protein 4 protein (12-235AA)
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Immunogen speciesHuman
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AliasPiwi-like protein 4,PIWIL4,HIWI2,PIWI
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BackgroundPlays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:12906857).
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ClonalityPolyclonal antibody
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Shipping and storagePIWIL4 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenPiwi-like protein 4
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolPIWIL4-AS1, PIWIL4
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Short nameAnti-PIWIL4
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Techniqueanti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates
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HostRabbit, Rabbits
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SpeciesHuman, Humans
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Alternative namePIWIL4 Antibody
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Alternative techniqueantibodies, rabbit-anti
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Alternative to gene targetpiwi-like 4 (Drosophila), PIWIL4 and IDBG-68329 and ENSG00000134627 and 143689, piRNA binding, nuclei, Piwil4 and IDBG-134536 and ENSMUSG00000036912 and 330890, PIWIL4 and IDBG-629500 and ENSBTAG00000014910 and 509545
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Gene info
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Identity
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Gene
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Long gene namePIWIL4 antisense RNA 1
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Locus
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Discovery year2021-03-12
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Entrez gene record
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi like RNA-mediated gene silencing 4
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Synonyms gene name
- piwi-like 4 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Piwi like RNA-mediated gene silencing family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data