Sign Arrays signal transduction
Roche LightCycler compatibility The LightCycler 480, for 96 well qPCR: XXXH1-RXS for 384 well: XXXH2-RXS. With perfect Master Mix Sybr Green: PMSX-WXS, 82 Euro
QPCR Master Mix We suppy PMS reagent or Perfect Master Mix SYBRG for SignArrays 96 at 10 μl of PMS per reaction for 20 μl final volume. The reference is: PMS1-Z2S, 82 euro
Genes All Pig Hematopoietic Stem Cells genes
Excel template We will supply you with a microsoft excel template to analyse the qPCR results and see the activation, or supression of genes of interestof your profiler.
Biorad qPCR cyclers The RT PCR CFX 96 cycler, CFX 384, CFX Connect, Opticon, Chomo, iQ and MyiQ Cycler are compatible withour Perfect Master Mix SYBR Green. Contact us for the reference.
Pathway profiling The expression RT PCR profiler array is supplied in 96 and 384 well primer plates. They are used to profile cDNA from human tissues, cells or body fluids. We can perform signaling pathways analysis with only 10-100 cells with the 2018 Direct lysis to SignArray reagent. For rare or precious sampleswe use the SpeAmpn reagents to analyze until 4 signaling pathways with only 5-50ng starting amount of RNA. The adapted hot start SYBR Green qPCR kit apropriate to your Real Time PCR cycler is supplied at 82$ or Euro. Please supply us with your qPCR cyler model when ordering.
Custom 96 or 384 well qPCR primers 1 euro or USD per pig gene added to the qPCR Hematopoietic Stem Cells 1 profiling plate.
Single gene qPCR Perfect Master Mix PROBE, PMP1-Z50 for 50 reactions, price: 75 eur/USD
AB cycler RT PCR blocks The Applied Biosystems Step One Plus Real-Time System 96 well is used with the qPCR XXXH1-FXS with Perfect Master Mix SYBR Green PMSX-RXS, 82 USD and ABI 7900 HT (standard block) XXXH1-AXS, XXXH2-AXS, PMSX-RXS and ViiA7 system (standard block), XXXH1-AXS, XXXH2-AXS, PMSX-LRXS and QuantStudio 6, 7, 12K Flex System (standard block)
Pathway profiling outsourcing We have a large collection of cell lines and can perfome the Hematopoietic Stem Cells 1 expression profiling for you. You can send us your tissues or we have a library of tissues and can perform the RNA extraction, make the cDNA and perform the RT-PCR and qPCR for you.
Distribution Gentaur ensures worldwide distribution of Pig Hematopoietic Stem Cells qPCR array except for spain
Sequence disclosure We do not standard disclose the sequence of our carefully selected and oprimized RT PCR primers.
Description More than 1 can be supplied by AnyGenes.
About Pigs and the smaller guinea pigs are frequent used as models for humans.
Test Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.