Phosphoprotein Purification test kit
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Catalog number
AKR-106
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Price
Please ask
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Size
5 preps
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We send this reagant on
Room temperature
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The temperatureerature for storage is
4ºC
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Technical file
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SDS or safty data sheet
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Type of reagent
Cell Signaling and Protein Biology
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Related techniques
Cell Signaling and Protein Biology, Protein Analysis Tools, Protein Phosphorylation, Phosphoprotein Purification test kit
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Use of the reagent
The Phosphoprotein Purification test kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
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Cellular use
Phosphorylated proteins are affinity purified from lysates with a single-step, purification matrix.
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Protocol information
Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).
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Reagent properties
Entire procedure takes about 4 hours
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,10
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Cellular biology labs supplies purification kits and ultra pure reagents.
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Gene target
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Short name
Phosphoprotein Purification test kit
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Technique
Purification, purified
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Alternative name
Phosphoprotein purification test reagent
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Alternative technique
purifications, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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